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pt7 spcas9 sgrna scaffold plasmid  (Addgene inc)


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    Structured Review

    Addgene inc pt7 spcas9 sgrna scaffold plasmid
    We tested Protein2PAM on 20 engineered Cas9 and Cas12 proteins with altered PAM specificities from 10 studies (Methods). These included variants of <t>SpCas9</t> ( , , ), SaCas9 , St1Cas9 , Nme2Cas9 , CjCas9 , and Cas12a ( , , ). In most cases, the model predicted the same PAMs as the wild-type counterparts with the exception of an Nme2Cas9 variant where Protein2PAM correctly predicted a shift from N 4 CC to N 4 CN.
    Pt7 Spcas9 Sgrna Scaffold Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt7 spcas9 sgrna scaffold plasmid/product/Addgene inc
    Average 93 stars, based on 3 article reviews
    pt7 spcas9 sgrna scaffold plasmid - by Bioz Stars, 2026-03
    93/100 stars

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    1) Product Images from "Engineering of CRISPR-Cas PAM recognition using deep learning of vast evolutionary data"

    Article Title: Engineering of CRISPR-Cas PAM recognition using deep learning of vast evolutionary data

    Journal: bioRxiv

    doi: 10.1101/2025.01.06.631536

    We tested Protein2PAM on 20 engineered Cas9 and Cas12 proteins with altered PAM specificities from 10 studies (Methods). These included variants of SpCas9 ( , , ), SaCas9 , St1Cas9 , Nme2Cas9 , CjCas9 , and Cas12a ( , , ). In most cases, the model predicted the same PAMs as the wild-type counterparts with the exception of an Nme2Cas9 variant where Protein2PAM correctly predicted a shift from N 4 CC to N 4 CN.
    Figure Legend Snippet: We tested Protein2PAM on 20 engineered Cas9 and Cas12 proteins with altered PAM specificities from 10 studies (Methods). These included variants of SpCas9 ( , , ), SaCas9 , St1Cas9 , Nme2Cas9 , CjCas9 , and Cas12a ( , , ). In most cases, the model predicted the same PAMs as the wild-type counterparts with the exception of an Nme2Cas9 variant where Protein2PAM correctly predicted a shift from N 4 CC to N 4 CN.

    Techniques Used: Variant Assay



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    pt7 spcas9 sgrna scaffold plasmid  (Addgene inc)
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    Addgene inc pt7 spcas9 sgrna scaffold plasmid
    We tested Protein2PAM on 20 engineered Cas9 and Cas12 proteins with altered PAM specificities from 10 studies (Methods). These included variants of <t>SpCas9</t> ( , , ), SaCas9 , St1Cas9 , Nme2Cas9 , CjCas9 , and Cas12a ( , , ). In most cases, the model predicted the same PAMs as the wild-type counterparts with the exception of an Nme2Cas9 variant where Protein2PAM correctly predicted a shift from N 4 CC to N 4 CN.
    Pt7 Spcas9 Sgrna Scaffold Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt7 spcas9 sgrna scaffold plasmid/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    pt7 spcas9 sgrna scaffold plasmid - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    msp3485  (Addgene inc)
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    We tested Protein2PAM on 20 engineered Cas9 and Cas12 proteins with altered PAM specificities from 10 studies (Methods). These included variants of <t>SpCas9</t> ( , , ), SaCas9 , St1Cas9 , Nme2Cas9 , CjCas9 , and Cas12a ( , , ). In most cases, the model predicted the same PAMs as the wild-type counterparts with the exception of an Nme2Cas9 variant where Protein2PAM correctly predicted a shift from N 4 CC to N 4 CN.
    Msp3485, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/msp3485/product/Addgene inc
    Average 90 stars, based on 1 article reviews
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    Image Search Results


    We tested Protein2PAM on 20 engineered Cas9 and Cas12 proteins with altered PAM specificities from 10 studies (Methods). These included variants of SpCas9 ( , , ), SaCas9 , St1Cas9 , Nme2Cas9 , CjCas9 , and Cas12a ( , , ). In most cases, the model predicted the same PAMs as the wild-type counterparts with the exception of an Nme2Cas9 variant where Protein2PAM correctly predicted a shift from N 4 CC to N 4 CN.

    Journal: bioRxiv

    Article Title: Engineering of CRISPR-Cas PAM recognition using deep learning of vast evolutionary data

    doi: 10.1101/2025.01.06.631536

    Figure Lengend Snippet: We tested Protein2PAM on 20 engineered Cas9 and Cas12 proteins with altered PAM specificities from 10 studies (Methods). These included variants of SpCas9 ( , , ), SaCas9 , St1Cas9 , Nme2Cas9 , CjCas9 , and Cas12a ( , , ). In most cases, the model predicted the same PAMs as the wild-type counterparts with the exception of an Nme2Cas9 variant where Protein2PAM correctly predicted a shift from N 4 CC to N 4 CN.

    Article Snippet: For in vitro transcription of gRNAs, the pT7-SpCas9-sgRNA-scaffold plasmid (MSP3485; Addgene ID 140082) was digested with NheI-HF and HindIII-HF (NEB) to remove the T7 promoter and SpCas9 gRNA scaffold.

    Techniques: Variant Assay